Isolamento e identificação de fungos endofiticos de Mikania laevigata (asteroceae) e análise química dos extratos por clae-dad

Abstract

The interactions between fungi and plants, especially endophytic fungi, are considered important sources of secondary metabolites. The detection of these microorganisms inside healthy plants has opened new perspectives for the study of fungus-plant interactions. This study aimed to isolate and identify the molecular analysis of endophytic fungi of Mikania laevigata (Asteraceae) and perform chemical analysis by HPLC-DAD extracts of these fungi. The leaves of Mikania laevigata grown in tree farm were subjected to the isolation procedure that resulted in endophytic fungi isolated 40. Of these, 15 were selected and characterized by morphological features as Xylariaceae ascomycetes family. Molecular investigation by sequencing the rDNA ITS region resulted in the identification of three strains of Nodulisporium sp., 3 strains of Hypoxylon sp. Daldinia sp 1 strain. and a species identified as Xylaria luteostromata. Could not be identified at the molecular level the other samples subjected to sequencing. The analysis by HPLC-DAD showed a large chemical diversity among the analyzed extracts. It was noted the presence of a greater amount of metabolites in the extracts compared to the external micellar extracts. The methanol extracts showed the presence of a greater number of metabolites when compared to extracts in ethyl acetate.