Diversidade e seleção de fungos basidiomicetos afiloforóides para produção de enzimas lignocelulolíticas em área de Mata Atlântica do sul da Bahia

Abstract

The capacity of basidiomycetes to degrade lignocellulosic compounds, such as wood, makes this target group of biotechnology research. The main enzymes related to lignocellulosic degradation compounds are laccase, lignin peroxidase (LiP), manganese peroxidase (MnP) and cellulases. The specimens collected in the Atlantic Forest of southern Bahia in the municipality of Ituberá in the preservation area of the Michelin Plantation of Brazil shown to be potential producers of these enzymes when subjected to screening tests. The fungus Fomitella supine showed the best potential for decolorization of Remazol Brilliant Blue R (RBBR) and degradation of carboxymethylcellulose (CMC) in the technique of "cup plate". The results of the optimization parameters showed that among the tested variables pH and nitrogen source were instrumental in the laccase activity. In culture conditions with pH 4.0, temperature 35 º C, with the nitrogen source yeast extract and glycerol carbon source selected fungus obtained the highest laccase activity of 250 UL-1.