Atividade antimicrobiana de microrganismos isolados de cupinzeiros da região da Mata de Cipó, Bahia

Abstract

The actinobacterias and Bacillus have been especially useful in the pharmaceutical industry for its ability to produce unlimited secondary metabolites with different chemical structures and biological activities. The search for new species of actinobacteria and species of genus Bacillus is an essential component to the discovery of new drugs. In traditional medicine, the termites are quite used for the treatment of various diseases in various parts of the world. The widespread use of drugs made based on termites and/or their nests and witness reports from its users about its effectiveness allow assume that most probably this effectiveness should be given to bioactive compounds produced by actinobacterias, since studies point to the presence of these bacteria associated with the microbial species of termites. This project aims to isolate microorganisms present in termite nest land of the region of the Cipó forest in the municipality of Lafaiete Coutinho, Feira de Santana e Morro do Chapéu - BA, aiming to check the antimicrobial potential of microorganisms. Were isolated 145microorganisms in the three localities sampled. Of these, 43 (29.65%) in municipality Lafaiete Coutinho, 55 (37.93%) in Morro do Chapéu and 47 (32.42%) in the municipality of Feira de Santana - BA. The antimicrobial activity evaluated solid medium was detected in 38 (26.20%) of total. In the city of Lafaiete Coutinho was observed in 14 (9.65%) in Morro do Chapéu 12 (8.27%) and Feira de Santana 12 (8.27%). Which, were antagonistic for S. aureus CCMB 262 (22.75%), for S. aureus CCMB 263 (20%), for S. aureus CCMB 285 (14,48%), for E. coli 284 (2,06%), for E. coli CCMB 261 (3,44%), 9.65% for S. choleraesuis CCMB 281 (9,65%), for C. albicans CCMB 266 (8,96%), for C. albicans CCMB 286 (12,41%) and for C. parapsilosis (10,34%). The 11 strains showing the best results in the first test were selected for characterization of the minimal inhibitory concentration (MIC). Of these, nine showed minimal inhibitory concentration in starch-casein agar. And of those, three were able to inhibit P. aeruginosa CCMB 268. Sequencing of 16S rDNA showed that the 11 sequenced isolates belong to the genus Streptomyces and Bacillus.