Avaliação in vitro do potencial de compostos naturais no processo de reparo tecidual

Abstract

Skin lesions are considered a public health problem that compromises the quality of life of patients. Research with the development of new therapies and technologies to accelerate the healing process is important given the high cost that wounds bring to the health system. In this sense, this work aimed to evaluate the potential of natural compounds in the tissue repair process through the Scratch assay using cultured L929 fibroblasts. In addition, the supernatant and cells from the fibroblast culture were used to determine the effects of the treatments on the production of important markers of the tissue repair process (IGF-1, VEGF, IL-8, IL-10, FGF2, COL1A2, COL4A, PDGF) by means of ELISA and qRT-PCR. The results showed that fraxetin, monnieriside A (MOA) and Patchouli essential oil were non-cytotoxic, with migration rates of 54.66%, 71.5% and 76%, respectively. These data were corroborated by marker analysis, as fraxetine was able to induce mRNA expression for biological markers IGF-1, VEGF, IL-8 and IL-10; MOA induced mRNA expression for FGF2, COL1A2 and IL-10; patchouli essential oil induced mRNA expression for IL-8 and IL-10, with p values <0.05. The compounds analyzed showed potential for the development of drugs indicated for promoting tissue repair. Research aimed at discovering new drugs to promote wound healing without intercurrences is necessary, in view of the importance of the subject for public health.