Síntese, caracterização e associação de nanopartículas de ouro com Bromelina

Abstract

The extract of the stem and green fruits from vegetables of the Bromeliaceae family (mainly pineapple - Ananas comosus) contains a set of proteolytic enzymes known generically as Bromelain, which are attributed with various pharmacological activities, such as healing and antiinflammatory action. , which can be improved with the help of nanomaterials. Nanotechnology has several forms of use in the pharmaceutical sector, including the use of nanoparticles that act as vehicles for the distribution of derived compounds. This study aimed to carry out a theoretical survey regarding the bromelain enzyme and gold nanoparticles (AuNPs), using this to begin practical research on the synthesis and characterization of gold nanoparticles associated with the bromelain enzyme. The nanoparticles produced showed a moderate stability of -27.8 mV, an average size of 47.6 nm and a polydispersity index of 0.391. After association, they reached values of -12 mV, 1.24 μm and 0.536, respectively. Microscopy showed that the nanoparticles produced were spherical and had an actual diameter of 20 nm after association. In the gold nanoparticles, bromelain was associated in approximately 77%, with an enzymatic activity of 23.36 U/mL, confirming the activity after adsorption. In comparison with the scientific literature, the data obtained suggests that the complex formed by gold nanoparticles and bromelain can be applied in healing films, through new studies.